| Title | Isolation and characterisation of arabinogalactan-proteins from New Zealand kanuka honey |
| Publication Type | Journal Article |
| Year of Publication | 2011 |
| Authors | Steinhorn, G., Sims I. M., Carnachan S. M., Carr A. J., and Schlothauer R. |
| IRL Team | Carbohydrate Chemistry |
| Journal | Food Chemistry |
| Volume | 128 |
| Pagination | 949-956 |
| Keywords | amino acid analysis, arabinogalactan, arabinogalactan protein, Arabinogalactan proteins, Arabinogalactan-protein, Arabinose, article, carbohydrate analysis, carbon nuclear magnetic resonance, Characterisation, Food products, galactose, galacturonic acid, Gel permeation chromatography, gel permeation chromatography multi angle laser light scattering, glucose, glucuronic acid, Glycosyl, heteronuclear multiple quantum coherence, honey, Kanuka, Laser light scattering, Light scattering, Linkage analysis, mannose, mass fragmentography, Molecular Weight, Neutral sugar, New Zealand, nuclear magnetic resonance spectroscopy, polymer, protein determination, protein isolation, Protein Structure, Proteins, proteoglycan, proton nuclear magnetic resonance, purification, SEC-MALLS, Size exclusion chromatography, Structural analysis, Sugars, Supernatant fractions, Trifolium, Type II, unclassified drug, xylose |
| Abstract | Fractionation of manuka, kanuka and clover honeys indicated the >10 kDa fraction contained small amounts of type II arabinogalactans (AGs), which are often present as arabinogalactan proteins (AGPs). AGPs were isolated from the >10 kDa fraction of kanuka honey using β-glucosyl Yariv reagent and their composition and structure analysed. Constituent sugar, glycosyl linkage and NMR[?] spectroscopy analysis of the purified AGP fraction revealed a predominance of neutral sugars, mainly galactose and arabinose, linked in a highly-branched structure typical of type II AGs. The molecular weight of the major component of the purified AGPs was ∼110 kDa, as determined by size-exclusion chromatography-multi-angle laser light scattering (SEC-MALLS). The Yariv supernatant fraction contained less total sugar, especially galactose, and more protein than purified AGPs. Linkage analysis indicated this fraction also contained an AG-type polymer in addition to various other polysaccharides and SEC-MALLS indicated the molecular weight of the major component was ∼32 kDa. © 2011 Elsevier Ltd. All rights reserved. |
| URL | http://www.scopus.com/inward/record.url?eid=2-s2.0-79958068750&partnerID=40&md5=388bfe965fa59ffc58e2f8a432be27b3 |
| DOI | 10.1016/j.foodchem.2011.03.124 |
